4Z28
Crystal structure of short hoefavidin biotin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.180, 61.180, 166.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.660 |
| R-factor | 0.18194 |
| Rwork | 0.180 |
| R-free | 0.23017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z27 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.791 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.045 | 0.798 |
| Number of reflections | 38372 | |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Streak seeding was applied with source crystals. Crystals appeared within 24 hours and grew to their maximal size within 2 weeks |
