4Z25
Mimivirus R135 (residues 51-702)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 169.974, 154.937, 197.391 |
| Unit cell angles | 90.00, 103.56, 90.00 |
Refinement procedure
| Resolution | 48.570 - 3.339 |
| R-factor | 0.1544 |
| Rwork | 0.151 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z24 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.554 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.570 | 3.460 |
| High resolution limit [Å] | 3.339 | 3.340 |
| Rmerge | 0.179 | 0.392 |
| Number of reflections | 141759 | |
| <I/σ(I)> | 9.2 | 4.4 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 8000, ammonium nitrate |
