4Z23
BspA_C_WT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 34.080, 206.210, 121.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.550 - 2.450 |
| R-factor | 0.18416 |
| Rwork | 0.181 |
| R-free | 0.24313 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.696 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.550 |
| High resolution limit [Å] | 2.450 |
| Number of reflections | 16205 |
| <I/σ(I)> | 3.19 |
| Completeness [%] | 98.9 |
| Redundancy | 12.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.02 M sodium/potassium phosphate and 20% (w/v) PEG 3350 |
