4Z21

Crystal structure of ARNO Sec7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97913
Spacegroup name	P 21 3
Unit cell lengths	86.630, 86.630, 86.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.742 - 2.050
R-factor	0.1928
Rwork	0.192
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jmi
RMSD bond length	0.009
RMSD bond angle	1.083
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.031	0.383
Number of reflections	13863
<I/σ(I)>	19.5	2.1
Completeness [%]	99.6	100
Redundancy	7	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M Bis-Tris pH 6.5, 25% PEG 3350