4Z1W
CRYSTAL STRUCTURE OF MONOMERIC BACTERIOPHYTOCHROME mutant D207L Y263F From Synchrotron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.434, 53.340, 65.690 |
| Unit cell angles | 90.00, 90.91, 90.00 |
Refinement procedure
| Resolution | 23.220 - 1.300 |
| R-factor | 0.14531 |
| Rwork | 0.145 |
| R-free | 0.16019 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s7q 4o8g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.314 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.800 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.350 | |
| Number of reflections | 77741 | |
| <I/σ(I)> | 33.2 | 1 |
| Completeness [%] | 96.5 | 94.3 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | Crystals of D207L-Y263F phytochrome mutant were grown from hanging drop vapor diffusion in 20% PEG400, starting protein concentration of 20 mg/ml, and 0.1M phosphate citrate buffer at pH 4.0. |
