4Z1P

BspA_C_mut

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-22
Detector	PSI PILATUS 6M
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	38.090, 48.240, 234.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.530 - 1.890
R-factor	0.19693
Rwork	0.195
R-free	0.24078
RMSD bond length	0.019
RMSD bond angle	1.817
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	58.530
High resolution limit [Å]	1.890
Number of reflections	35603
<I/σ(I)>	2.65
Completeness [%]	99.3
Redundancy	12.25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MMT buffer (1:2:2 L-Malic acid, MES, Tris [pH 6.0]) and 25% (w/v) PEG1500