4Z1O
Hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRT) from Sulfolobus solfataricus in complex with alpha-phosphoribosylpyrophosphoric acid (PRPP) and Magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9395 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.784, 73.784, 142.906 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.136 - 2.150 |
R-factor | 0.1932 |
Rwork | 0.189 |
R-free | 0.27870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vdm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.031 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 | |
High resolution limit [Å] | 2.150 | 6.390 | 2.150 |
Rmerge | 0.185 | 0.093 | 0.867 |
Rmeas | 0.191 | 0.096 | 0.891 |
Total number of observations | 411125 | ||
Number of reflections | 22230 | 967 | 3433 |
<I/σ(I)> | 12.35 | 27.03 | 3.12 |
Completeness [%] | 99.6 | 99.2 | 98.1 |
Redundancy | 18.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES, 15% PEG3350 |