4Z1I
Crystal structure of human Trap1 with AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 |
Unit cell lengths | 115.547, 115.547, 339.936 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.822 - 3.300 |
R-factor | 0.2231 |
Rwork | 0.220 |
R-free | 0.28470 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.679 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.300 |
Number of reflections | 38253 |
<I/σ(I)> | 2.5 |
Completeness [%] | 99.0 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 23% PEG 4K, 100mM Tris, 0.35M ammonium sulfate. |