4Z1H
Crystal structure of human Trap1 with SMTIN-P01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.336, 69.336, 255.774 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.354 - 2.900 |
R-factor | 0.2382 |
Rwork | 0.235 |
R-free | 0.28530 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.987 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 14775 |
<I/σ(I)> | 23.4 |
Completeness [%] | 99.8 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 16% PEG 8K, 100mM calcium acetate, 100mM sodium cacodylate. |