4Z17

Thermostable enolase from Chloroflexus aurantiacus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.95369
Spacegroup name	I 4
Unit cell lengths	146.279, 146.279, 101.777
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.680 - 2.650
R-factor	0.1953
Rwork	0.193
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iyx
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.680	2.790
High resolution limit [Å]	2.650	2.650
Rmerge	0.073	0.561
Number of reflections	31118
<I/σ(I)>	3.2	3.2
Completeness [%]	99.6	99.4
Redundancy	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	291	Bis-Tris propane buffer (0.1 M, pH 9.0), 0.21 M NaCl and 28% PEG 1500, 20% (v/v) glycerol