4Z17
Thermostable enolase from Chloroflexus aurantiacus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-16 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.95369 |
Spacegroup name | I 4 |
Unit cell lengths | 146.279, 146.279, 101.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.680 - 2.650 |
R-factor | 0.1953 |
Rwork | 0.193 |
R-free | 0.23780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iyx |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.680 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.073 | 0.561 |
Number of reflections | 31118 | |
<I/σ(I)> | 3.2 | 3.2 |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9 | 291 | Bis-Tris propane buffer (0.1 M, pH 9.0), 0.21 M NaCl and 28% PEG 1500, 20% (v/v) glycerol |