4Z14
Recombinantly expressed latent aurone synthase (polyphenol oxidase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0247 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.850, 103.790, 175.560 |
| Unit cell angles | 100.69, 91.90, 105.18 |
Refinement procedure
| Resolution | 47.543 - 2.530 |
| R-factor | 0.2103 |
| Rwork | 0.208 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z0y |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.625 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.910 | 2.620 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.134 | 0.965 |
| Number of reflections | 136987 | |
| <I/σ(I)> | 3.6 | 0.8 |
| Completeness [%] | 97.9 | 96.71 |
| Redundancy | 1.97 | 2.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15 % PEG 4000, 100 mM magnesium chloride, 60 mM sodium citrate, pH 7.4 |
