4Z0U

RNase HI/SSB-Ct complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 2 2 21
Unit cell lengths	60.498, 64.855, 79.726
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.000
R-factor	0.21262
Rwork	0.211
R-free	0.24089
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.071
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.059	0.025	0.575
Rmeas	0.068	0.029	0.667
Rpim	0.033	0.015	0.334
Total number of observations	88106
Number of reflections	21970
<I/σ(I)>	11.5
Completeness [%]	99.9	98.9	99.6
Redundancy	4	3.6	3.9
CC(1/2)		0.999	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	sodium citrate, HEPES, glycerol, 1,4 dioxane