4Z0S
F96A Mutant of Plasmodium Falciparum Triosephosphate Isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.420, 76.840, 76.570 |
| Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
| Resolution | 53.870 - 2.390 |
| R-factor | 0.22992 |
| Rwork | 0.228 |
| R-free | 0.27122 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m7p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.120 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.870 | 2.490 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.210 | |
| Number of reflections | 17068 | |
| <I/σ(I)> | 17 | 5.4 |
| Completeness [%] | 92.0 | 70 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | NaOAc, 10mM Li2SO4, 0.5mM DTT, 0.5mM NaN3, 0.5mM EDTA, 24% PEG 1450 |
