4Z0O

CW-type zinc finger of ZCWPW2 with F78D mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-21
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 3 2 1
Unit cell lengths	47.569, 47.569, 48.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.570
R-factor	0.1648
Rwork	0.164
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o62
RMSD bond length	0.017
RMSD bond angle	1.729
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.520	31.520	1.600
High resolution limit [Å]	1.570	8.610	1.570
Rmerge	0.032	0.020	0.203
Rpim	0.011	0.007	0.068
Total number of observations	93278	554	4108
Number of reflections	9271
<I/σ(I)>	56.2	124.8	13.3
Completeness [%]	99.8	97.8	97.4
Redundancy	10.1	7.6	9.5
CC(1/2)	1.000	1.000	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2 M ammonium sulfate, 2% PEG400, 0.1 M HEPES sodium