4Z0N
Crystal Structure of a Periplasmic Solute binding protein (IPR025997) from Streptobacillus moniliformis DSM-12112 (Smon_0317, TARGET EFI-511281) with bound D-Galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.396, 44.011, 62.037 |
| Unit cell angles | 90.00, 104.32, 90.00 |
Refinement procedure
| Resolution | 24.115 - 1.260 |
| R-factor | 0.1399 |
| Rwork | 0.139 |
| R-free | 0.16500 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.039 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.260 |
| Rmerge | 0.092 |
| Total number of observations | 301 |
| Number of reflections | 73384 |
| <I/σ(I)> | 8 |
| Completeness [%] | 88.6 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | Protein (20 mM HEPES, pH 7.5, 5 mM DTT, 10 mM D-Galactose); Reservoir (0.1 M Sodium acetate:HCl pH 4.5, 2.0 M Ammonium sulfate); Cryoprotection (80% 2M Lithium sulfate, 20% Reservoir |
