4Z0M
EchA5 Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 136.708, 136.708, 125.802 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.530 - 1.970 |
| R-factor | 0.16925 |
| Rwork | 0.168 |
| R-free | 0.19455 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qka |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.104 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.530 | 2.017 |
| High resolution limit [Å] | 1.970 | 1.966 |
| Rmerge | 0.200 | 0.230 |
| Number of reflections | 96574 | |
| <I/σ(I)> | 7.9 | 1.6 |
| Completeness [%] | 99.3 | 96.2 |
| Redundancy | 10.8 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277.15 | 0.2 M Ammonium sulfate, 0.1M Sodium acetate trihydrate, 30%(w/v) Polyethylene glycol monomethyl ether 2000 |
