4Z0H
X-ray structure of cytoplasmic glyceraldehyde-3-phosphate dehydrogenase (GapC1) complexed with NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 76.849, 76.849, 406.626 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.770 - 2.300 |
| R-factor | 0.2508 |
| Rwork | 0.249 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2b |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.940 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.660 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.129 | 0.454 |
| Number of reflections | 38786 | |
| <I/σ(I)> | 8.4 | 2.2 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 8.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 3.0-3.5 M ammonium sulphate, 0.1 M Na-Hepes. |
