4Z0C

Crystal structure of TLR13-ssRNA13 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-30
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	112.549, 115.209, 167.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.900 - 2.300
Rwork	0.191
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	89687
<I/σ(I)>	27.5
Completeness [%]	92.9
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1%(w/v) tryptone, 0.05M HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) sodium pH 7.0 and 12%(v/v) polyethylene glycol (PEG) 3,350