4YZO
Crystal Structure Analysis of Thiolase-like protein, ST0096 from Sulfolobus Tokodaii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2007-04-02 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97895, 0.97947, 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.763, 80.863, 137.512 |
| Unit cell angles | 90.00, 110.80, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.700 |
| R-factor | 0.16359 |
| Rwork | 0.162 |
| R-free | 0.19435 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.096 | 0.271 |
| Number of reflections | 180973 | |
| <I/σ(I)> | 16.7 | 2.8 |
| Completeness [%] | 96.7 | 78.3 |
| Redundancy | 5.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 293 | 30 % 2-ethoxyethanol, 50 mM Calcium Acetate, 0.1 M Imidazole, pH 8.0 |
