4YZI

Crystal structure of blue-shifted channelrhodopsin mutant (T198G/G202A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-16
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	59.640, 142.420, 92.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.143 - 2.500
R-factor	0.2238
Rwork	0.223
R-free	0.24100
RMSD bond length	0.003
RMSD bond angle	0.726
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	13967
<I/σ(I)>	11.8
Completeness [%]	99.9
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293	PEG 500DME, HEPES-NaOH pH 7.0, Li2SO4, ATP