4YZ1
Crystal Structure of Streptococcus pneumoniae NanC, apo structure.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9253 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 150.134, 73.736, 136.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.050 - 1.970 |
| R-factor | 0.18295 |
| Rwork | 0.181 |
| R-free | 0.21519 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jkb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.643 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 136.100 | 1.990 |
| High resolution limit [Å] | 1.969 | 1.970 |
| Rmerge | 0.099 | 0.533 |
| Number of reflections | 107569 | |
| <I/σ(I)> | 13.9 | 1.93 |
| Completeness [%] | 98.6 | 99.5 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG3350, 0.25M Ammonium sulfate, 0.1M pH8, 7.5% Ethanol |
