4YYR
Ficin B crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.902, 89.902, 55.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.049 - 1.349 |
| R-factor | 0.1339 |
| Rwork | 0.133 |
| R-free | 0.15370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yyq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.049 | 1.390 |
| High resolution limit [Å] | 1.349 | 1.350 |
| Rmerge | 0.088 | 0.374 |
| Number of reflections | 53284 | |
| <I/σ(I)> | 18.1 | 3.3 |
| Completeness [%] | 94.7 | 66.6 |
| Redundancy | 8.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M AS, 0.1 M Na HEPES 7.5, 2% PEG400 |
