4YYJ
Crystal structure of BRD9 Bromodomain bound to a butyryllysine peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 |
| Unit cell lengths | 24.823, 34.331, 129.067 |
| Unit cell angles | 90.86, 90.00, 111.19 |
Refinement procedure
| Resolution | 32.260 - 1.850 |
| R-factor | 0.2008 |
| Rwork | 0.198 |
| R-free | 0.24360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hme |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.850 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.077 | 0.055 | 0.370 |
| Total number of observations | 64982 | ||
| Number of reflections | 32888 | ||
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 97.4 | 97.9 | 95.9 |
| Redundancy | 2 | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M ammonium chloride, 20% w/v PEG3350 |
