4YYF

The crystal structure of a glycosyl hydrolase of GH3 family member from [Mycobacterium smegmatis str. MC2 155

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	103.067, 130.941, 85.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.410 - 1.920
R-factor	0.1805
Rwork	0.179
R-free	0.21010
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.110
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.500	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.093	0.771
Number of reflections	89349
<I/σ(I)>	23.3	2.6
Completeness [%]	100.0	100
Redundancy	6.1	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	2.8M Sodium Acetate