4YY1
The structure of hemagglutinin from a H6N1 influenza virus (A/chicken/Taiwan/A2837/2013) in complex with human receptor analog 6'SLNLN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 3 |
Unit cell lengths | 97.060, 97.060, 131.649 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.540 - 3.100 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.966 |
Data reduction software | SCALA |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.096 | 3.100 |
Rmerge | 0.118 | 0.812 |
Number of reflections | 23294 | |
<I/σ(I)> | 8.5 | 1.5 |
Completeness [%] | 92.6 | 93.9 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M sodium thiocyanate, 20% w/v polyethylene glycol 3350 |