4YY0
The structure of hemagglutinin from a H6N1 influenza virus (A/chicken/Taiwan/A2837/2013)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.610, 106.257, 125.277 |
| Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
| Resolution | 41.370 - 2.590 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.489 |
| Data reduction software | SCALA |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Rmerge | 0.115 | 0.872 |
| Number of reflections | 53268 | |
| <I/σ(I)> | 12.5 | 1.8 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M sodium thiocyanate, 20% w/v polyethylene glycol 3350 |
