4YXG
F96Y Mutant of Plasmodium Falciparum Triosephosphate Isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.000, 70.320, 72.580 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 35.510 - 1.900 |
| R-factor | 0.18257 |
| Rwork | 0.180 |
| R-free | 0.22432 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vfh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.034 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.225 | |
| Number of reflections | 28683 | |
| <I/σ(I)> | 15 | 4.3 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 16% PEG 1450, HEPES, 10mM CaCl2, 0.5mM DTT, 0.5mM NaN3, 0.5mM EDTA |
