4YXB

FliM(SPOA)::FliN fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	75.150, 81.500, 89.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.078 - 2.560
R-factor	0.2218
Rwork	0.217
R-free	0.25930
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.307
Data scaling software	Aimless (0.2.7)
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.670	57.670	2.670
High resolution limit [Å]	2.560	8.870	2.560
Rmerge	0.097	0.058	1.215
Rpim	0.027	0.017	0.332
Total number of observations	254307	6186	31740
Number of reflections	18420
<I/σ(I)>	18.5	47.2	2.7
Completeness [%]	100.0	98.7	100
Redundancy	13.8	12.1	14.3
CC(1/2)	0.999	0.998	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	FliM(245-334)::FliN(5-137) was concentrated to 7.5mg/mL and crystallized with 2.2M NaCl, 100mM imidazole.Cl pH=8.0. Crystals were cryoprotected with 2M NaCl, 100mM imidazole.Cl pH=8.0, 30% glycerol.