4YWZ
Crystal structure of the extracellular receptor domain of the essential sensor kinase WalK from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-04-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 32 |
| Unit cell lengths | 66.701, 66.701, 80.492 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.16632 |
| Rwork | 0.165 |
| R-free | 0.19667 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.205 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | RESOLVE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 44089 |
| <I/σ(I)> | 41.4 |
| Completeness [%] | 100.0 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 289 | 0.2 M ammonium citrate dibasic, 20% w/v polyethylene glycol 3350, pH 5.1 |
