4YWT
Crystal structure of full-length glypican-1 core protein after controlled crystal dehydration to 87% relative humidity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.780, 166.590, 137.700 |
| Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
| Resolution | 43.223 - 2.380 |
| R-factor | 0.2398 |
| Rwork | 0.239 |
| R-free | 0.27280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bwe |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.856 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.220 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.108 | 0.690 |
| Number of reflections | 82475 | |
| <I/σ(I)> | 7.44 | 1.62 |
| Completeness [%] | 97.9 | 97.11 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG 6000, Tris-HCl and CaCl2 |
