4YWM
Pyrococcus furiosus MCM N-terminal domain beta-turn triple mutant pentameric ring
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 210.709, 210.709, 209.638 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.200 |
R-factor | 0.23902 |
Rwork | 0.237 |
R-free | 0.26962 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pof |
RMSD bond length | 0.012 |
RMSD bond angle | 1.440 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.640 | |
Number of reflections | 88667 | |
<I/σ(I)> | 18 | 3.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 290 | 100 mM Bis-Tris, pH 5.5, 1.75 M ammonium sulfate |