4YWL
Pyrococcus furiosus MCM N-terminal domain F179A point mutant pentameric ring
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 213.018, 213.018, 213.978 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.20367 |
| Rwork | 0.202 |
| R-free | 0.22988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pof |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.183 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 1.000 | |
| Number of reflections | 92894 | |
| <I/σ(I)> | 13 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.4 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 50 mM sodium cacodylate, pH 6.5, 1 mM spermine, 35 mM MgCl2, 2.45 M ammonium sulfate |
