4YWK
Pyrococcus furiosus MCM N-terminal domain with Zinc-binding subdomain B deleted
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-10-12 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 41.958, 50.964, 52.880 |
Unit cell angles | 96.54, 94.77, 95.45 |
Refinement procedure
Resolution | 50.000 - 1.550 |
R-factor | 0.21882 |
Rwork | 0.217 |
R-free | 0.24994 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pof |
RMSD bond length | 0.010 |
RMSD bond angle | 1.450 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.082 | |
Number of reflections | 60090 | |
<I/σ(I)> | 32 | 13 |
Completeness [%] | 96.2 | 94.5 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 290 | 50 mM ammonium fluoride, 21% PEG 3350 |