4YWI
F96S/L167V Double mutant of Plasmodium Falciparum Triosephosphate Isomerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-06-30 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 50.933, 53.809, 174.495 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.890 - 1.850 |
R-factor | 0.16383 |
Rwork | 0.162 |
R-free | 0.20294 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m7p |
RMSD bond length | 0.008 |
RMSD bond angle | 1.248 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.930 | 1.940 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.076 | 0.180 |
Number of reflections | 35109 | |
<I/σ(I)> | 38 | 12.8 |
Completeness [%] | 83.6 | 61.4 |
Redundancy | 2.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 1450 22%, HEPES, Li2SO4 10mM, DTT 0.5mM, NaN3 0.5mM, EDTA 0.5mM |