4YWF
AbyA5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97957 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.080, 87.180, 47.910 |
Unit cell angles | 90.00, 101.63, 90.00 |
Refinement procedure
Resolution | 36.390 - 2.000 |
R-factor | 0.21883 |
Rwork | 0.218 |
R-free | 0.23643 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.817 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.390 | |
High resolution limit [Å] | 2.000 | |
Rmerge | 0.065 | 0.290 |
Number of reflections | 25075 | |
<I/σ(I)> | 3.4 | |
Completeness [%] | 99.9 | |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.02M magnesium chloride, 0.1M HEPES, 22% w/v polyacrylic acid 5100 sodium salt, pH 7.5 |