4YWC
Crystal structure of Myc3(5-242) fragment in complex with Jaz9(218-239) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.105, 110.627, 161.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.621 - 2.400 |
| R-factor | 0.2417 |
| Rwork | 0.239 |
| R-free | 0.29510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rru |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.244 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.139 | 1.070 |
| Number of reflections | 21208 | |
| <I/σ(I)> | 10.3 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.2 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 293 | The purified proteins at a concentration of 15 mg/ml are mixed with 0.2 M magnesium nitrate, 20% (w/v) polyethylene glycol 3,350. Crystals of about 100 um in length appeared in 3 days |
