4YW9
Structure of rat cytosolic pepck in complex with 3-mercaptopicolinic acid and GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.407, 118.742, 60.248 |
| Unit cell angles | 90.00, 110.02, 90.00 |
Refinement procedure
| Resolution | 29.160 - 1.400 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qew |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.787 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.058 | 0.417 |
| Number of reflections | 110377 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 94.4 | 76.2 |
| Redundancy | 7.2 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 25% PEG 3350, 0.1M HEPES PH 7.4,2 MM MNCL2, 10MM GTP, 1MM 3-MERCAPTOPICOLINIC ACID, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
