4YVQ
Crystal Structure of FLU-TPR in Complex with the C-terminal Region of GluTR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 74.735, 74.735, 161.659 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.280 - 2.401 |
R-factor | 0.2095 |
Rwork | 0.208 |
R-free | 0.24210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yvo |
RMSD bond length | 0.004 |
RMSD bond angle | 0.675 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 11100 |
<I/σ(I)> | 26.6 |
Completeness [%] | 99.9 |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 277 | 0.15M KBr, 30% w/v Polyethylene glycol monomethyl ether 2000 |