4YVQ

Crystal Structure of FLU-TPR in Complex with the C-terminal Region of GluTR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 65 2 2
Unit cell lengths	74.735, 74.735, 161.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.280 - 2.401
R-factor	0.2095
Rwork	0.208
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yvo
RMSD bond length	0.004
RMSD bond angle	0.675
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	11100
<I/σ(I)>	26.6
Completeness [%]	99.9
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	0.15M KBr, 30% w/v Polyethylene glycol monomethyl ether 2000