4YVO

Crystal Structure of the TPR Domain of Arabidopsis FLU (FLU-TPR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	60.193, 60.193, 67.489
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.064 - 1.450
R-factor	0.1421
Rwork	0.140
R-free	0.17540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ro3
RMSD bond length	0.005
RMSD bond angle	0.942
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.450
Number of reflections	25072
<I/σ(I)>	24.9
Completeness [%]	98.0
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	0.2M NaCl, 0.1M Bis-tris pH6.5, 25% PEG 3350