4YVM
X-ray structure of Helicobacter pylori CagL-K74
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97938 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 97.616, 97.616, 144.242 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.346 - 2.791 |
R-factor | 0.2024 |
Rwork | 0.199 |
R-free | 0.26710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Se-met K74 CagL |
RMSD bond length | 0.009 |
RMSD bond angle | 1.077 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.791 | 2.800 |
Rmerge | 0.110 | 0.330 |
Number of reflections | 17703 | |
<I/σ(I)> | 14 | 2.64 |
Completeness [%] | 98.5 | 94.6 |
Redundancy | 6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M Na-citrate, 10% iso-propanol, 10% PEG 4000, 3% dioxane |