4YVM

X-ray structure of Helicobacter pylori CagL-K74

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97938
Spacegroup name	P 43 21 2
Unit cell lengths	97.616, 97.616, 144.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.346 - 2.791
R-factor	0.2024
Rwork	0.199
R-free	0.26710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Se-met K74 CagL
RMSD bond length	0.009
RMSD bond angle	1.077
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.791	2.800
Rmerge	0.110	0.330
Number of reflections	17703
<I/σ(I)>	14	2.64
Completeness [%]	98.5	94.6
Redundancy	6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1M Na-citrate, 10% iso-propanol, 10% PEG 4000, 3% dioxane