4YVH

Crystal Structure of H. influenzae TrmD in complex with sinefungin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	93.766, 93.766, 177.907
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.241 - 1.600
R-factor	0.1876
Rwork	0.186
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uaj
RMSD bond length	0.014
RMSD bond angle	1.538
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	39872
<I/σ(I)>	30.2
Completeness [%]	99.8
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium citrate, CHES