4YVD
Crytsal structure of human Pleiotropic Regulator 1 (PRL1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-29 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 158.330, 34.185, 48.818 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.550 - 1.700 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gnq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.7) |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.104 | 0.874 |
| Number of reflections | 29598 | |
| <I/σ(I)> | 11.7 | 2.2 |
| Completeness [%] | 97.1 | 81.5 |
| Redundancy | 7.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2 M CaCl |
