4YVB
Structure of D128N streptavidin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-01-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.597, 98.950, 52.638 |
Unit cell angles | 90.00, 112.58, 90.00 |
Refinement procedure
Resolution | 33.970 - 1.351 |
R-factor | 0.1289 |
Rwork | 0.127 |
R-free | 0.17020 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1mep |
RMSD bond length | 0.018 |
RMSD bond angle | 1.770 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.672 | 34.672 | 1.420 |
High resolution limit [Å] | 1.351 | 4.270 | 1.350 |
Rmerge | 0.016 | 1.096 | |
Rmeas | 0.048 | ||
Rpim | 0.022 | 0.008 | 0.564 |
Total number of observations | 459497 | 15031 | 64197 |
Number of reflections | 101243 | ||
<I/σ(I)> | 16.6 | 60.7 | 1.3 |
Completeness [%] | 97.0 | 98.3 | 93.9 |
Redundancy | 4.5 | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.05 M HEPES, pH 7.5 with 7.5% PEG 8000 |