4YVA

Cathepsin K co-crystallized with actinomycetes extract

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-02-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97
Spacegroup name	P 43 21 2
Unit cell lengths	56.720, 56.720, 129.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.980 - 1.800
R-factor	0.184
Rwork	0.183
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c9e
RMSD bond length	0.004
RMSD bond angle	0.801
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.981	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.071	0.495
Number of reflections	20402
<I/σ(I)>	18.2	4.2
Completeness [%]	99.7	99.5
Redundancy	9.8	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294.15	24% PEG 8000, 0.2M Ammonium sulfate