4YV9

X-ray crystal structure of Streptococcus dysgalactiae SHP pheromone receptor Rgg2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97900
Spacegroup name	P 1 21 1
Unit cell lengths	68.388, 99.427, 99.882
Unit cell angles	90.00, 97.00, 90.00

Refinement procedure

Resolution	39.140 - 1.950
R-factor	0.1888
Rwork	0.188
R-free	0.21320
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	96464
<I/σ(I)>	45.25
Completeness [%]	99.8	100
Redundancy	7.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	CsA, Tris-HCl (pH 8.0), sodium phosphate, NaCl, DTT, DMSO, Ammonium sulfate