4YV6
X-ray crystal structure of Streptococcus dysgalactiae SHP pheromone receptor Rgg2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0750 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.103, 99.076, 99.895 |
Unit cell angles | 90.00, 97.03, 90.00 |
Refinement procedure
Resolution | 39.956 - 2.053 |
R-factor | 0.2086 |
Rwork | 0.208 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.523 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 87750 | |
<I/σ(I)> | 29.68 | |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 3.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | Tris-HCl (pH 8.0), sodium phosphate (pH 8.0), NaCl, DTT, 2.4 M Ammonium sulfate, and Tris-HCl (pH 8.6) |