4YV3
Trimeric crystal structure of vimentin coil1B fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.060, 60.950, 48.900 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 47.379 - 2.000 |
| R-factor | 0.2568 |
| Rwork | 0.255 |
| R-free | 0.29620 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.487 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 | |
| High resolution limit [Å] | 2.000 | 9.000 | 2.000 |
| Rmerge | 0.091 | 0.054 | 0.895 |
| Rmeas | 0.098 | 0.059 | 0.966 |
| Total number of observations | 219408 | ||
| Number of reflections | 29510 | 299 | 4024 |
| <I/σ(I)> | 11.98 | 27.4 | 2.25 |
| Completeness [%] | 99.7 | 91.7 | 99.2 |
| Redundancy | 7.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 10mg/ml protein in 10mM Tris-HCl pH 8, 38 mM NaCl mixed in v/v ratio 1:1 with ammonium sulphate 2.2M, sodium thiocyanate 0.2 M |
