4YUS

Crystal structure of photoactivated adenylyl cyclase of a cyanobacteriaOscillatoria acuminata in hexagonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.95
Spacegroup name	P 61 2 2
Unit cell lengths	76.838, 76.838, 204.778
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.480 - 1.800
R-factor	0.2147
Rwork	0.212
R-free	0.26430
RMSD bond length	0.008
RMSD bond angle	1.178
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	33448
<I/σ(I)>	8.7
Completeness [%]	97.7
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M sodium citrate pH5.0, 10% PEG 20K, 5mM Mgnasium chloride, 5mM ApCpp