4YUO
High-resolution multiconformer synchrotron model of CypA at 273 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2013-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.900, 52.430, 89.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.188 - 1.200 |
| R-factor | 0.1277 |
| Rwork | 0.127 |
| R-free | 0.14630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cpl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.188 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.112 | 0.204 |
| Number of reflections | 58118 | |
| <I/σ(I)> | 10.99 | 5.93 |
| Completeness [%] | 91.0 | 82 |
| Redundancy | 5.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Crystals were grown by mixing equal volumes of well solution (100 mM HEPES pH 7.5, 23% PEG 3350, 5 mM TCEP) and protein (60 mg/mL in 20 mM HEPES pH 7.5, 100 mM NaCl, 0.5 mM TCEP) in the hanging-drop format. |
