4YU4

Crystal structure of Mongoose (Helogale parvula) hemoglobin at pH 7.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-02-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5417
Spacegroup name	P 21 21 21
Unit cell lengths	55.920, 88.210, 129.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.1658
Rwork	0.163
R-free	0.22695
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rao
RMSD bond length	0.016
RMSD bond angle	1.663
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Number of reflections	16199
<I/σ(I)>	10.8
Completeness [%]	98.8
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	PEG 3350